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Plant traits are often measured in the field or laboratory to characterize stress responses. However, direct measurements are not always cost effective for broader sampling efforts, whereas indirect approaches such as reflectance spectroscopy could offer efficient and scalable alternatives. Here, we used field spectroscopy to assess whether (1) existing vegetation indices could predict leaf trait responses to heat stress, or if (2) partial least squares regression (PLSR) spectral models could quantify these trait responses. On several warm, sunny days, we measured leaf trait responses indicative of photosynthetic mechanisms, plant water status, and morphology, including electron transport rate (ETR), photochemical quenching (qP), leaf water potential (Ψleaf), and specific leaf area (SLA) in 51 urban trees from nine species. Concurrent measures of hyperspectral leaf reflectance from the same individuals were used to calculate vegetation indices for correlation with trait responses. We found that vegetation indices predicted only SLA robustly (R2 = 0.55), while PLSR predicted all leaf trait responses of interest with modest success (R2 = 0.36 to 0.58). Using spectral band subsets corresponding to commercially available drone-mounted hyperspectral cameras, as well as those selected for use in common multispectral satellite missions, we were able to estimate ETR, qP, and SLA with reasonable accuracy, highlighting the potential for large-scale prediction of these parameters. Overall, reflectance spectroscopy and PLSR can identify wavelengths and wavelength ranges that are important for remote sensing-based modeling of important functional trait responses of trees to heat stress over broad ranges. 

Abstract. Accurate assessment of leaf functional traits is crucial for a diverse range of applications from crop phenotyping to parameterizing global climate models. Leaf reflectance spectroscopy offers a promising avenue to advance ecological and agricultural research by complementing traditional, time-consuming gas exchange measurements. However, the development of robust hyperspectral models for predicting leaf photosynthetic capacity and associated traits from reflectance data has been hindered by limited data availability across species and environments. Here we introduce the Global Spectra-Trait Initiative (GSTI), a collaborative repository of paired leaf hyperspectral and gas exchange measurements from diverse ecosystems. The GSTI repository currently encompasses over 7500 observations from 397 species and 41 sites gathered from 36 published and unpublished studies, thereby offering a key resource for developing and validating hyperspectral models of leaf photosynthetic capacity. The GSTI database is developed on GitHub (https://github.com/plantphys/gsti, last access: 4 January 2026) and published to ESS-DIVE https://doi.org/10.15485/2530733, Lamour et al., 2025). It includes gas exchange data, derived photosynthetic parameters, and key leaf traits often associated with traditional gas exchange measurements such as leaf mass per area and leaf elemental composition. By providing a standardized repository for data sharing and analysis, we present a critical step towards creating hyperspectral models for predicting photosynthetic traits and associated leaf traits for terrestrial plants.